Journal: Bioscience, biotechnology, and biochemistry

Article Title: Inhibition of miR-21-5p suppresses high glucose-induced proliferation and angiogenesis of human retinal microvascular endothelial cells by the regulation of AKT and ERK pathways via maspin.

doi: 10.1080/09168451.2018.1459179

Figure Lengend Snippet: Figure 1. Effects of high glucose on cell proliferation and miR-21-5p, VEGF, VEGFR2 expression of HRMECs. Cell proliferation was measured by MTT assay (a). The level of miR-21-5p was detected by real-time PCR (b). The mRNA levels of VEGF (c) and VEGFR2 (e) were measured by real-time PCR. The protein levels of VEGF (d) and VEGFR2 (f) were determined by western blot. Experiments were repeated at least for three times. **p<0.05, **p<0.01 vs. control cells, ##p<0.01 vs. 24 h treated cells.

Article Snippet: Human retinal microvascular endothelial cells (HRMECs) were purchased from Angio-Proteomie (Boston, MA, USA) and cultured in endothelial cell medium (ECM, Sciencell) supplemented with 5% fetal bovine serum (FBS, Sciencell), 100 U/mL penicillin and 100 μg/mL streptomycin at 37 °C under 5% CO2 and 95% ambient air.

Techniques: Expressing, MTT Assay, Real-time Polymerase Chain Reaction, Western Blot, Control

Journal: Bioscience, biotechnology, and biochemistry

Article Title: Inhibition of miR-21-5p suppresses high glucose-induced proliferation and angiogenesis of human retinal microvascular endothelial cells by the regulation of AKT and ERK pathways via maspin.

doi: 10.1080/09168451.2018.1459179

Figure Lengend Snippet: Figure 2. Effects of miR-21-5p on the proliferation of HRMECs. Cell proliferation was detected by MTT assay (a). Expression of Ki67 was measured by immunofluorescence (b, c). Typical images from three repeats were shown. **p<0.05, **p<0.01 vs. control group, #p<0.05, ##p<0.01 vs. high glucose + NC inhibitor-treated cells. NC: negative control.

Article Snippet: Human retinal microvascular endothelial cells (HRMECs) were purchased from Angio-Proteomie (Boston, MA, USA) and cultured in endothelial cell medium (ECM, Sciencell) supplemented with 5% fetal bovine serum (FBS, Sciencell), 100 U/mL penicillin and 100 μg/mL streptomycin at 37 °C under 5% CO2 and 95% ambient air.

Techniques: MTT Assay, Expressing, Immunofluorescence, Control, Negative Control

Journal: Bioscience, biotechnology, and biochemistry

Article Title: Inhibition of miR-21-5p suppresses high glucose-induced proliferation and angiogenesis of human retinal microvascular endothelial cells by the regulation of AKT and ERK pathways via maspin.

doi: 10.1080/09168451.2018.1459179

Figure Lengend Snippet: Figure 3. Effects of miR-21-5p on the angiogenesis of HRMECs. Cell migration was detected by wound healing assay (a, b). Expression of MMP-2 and MMP-9 were measured by western blot (c). Tube formation and branching points were determined (d, e). Expression of HIF-1α and VEGF were measured by western blot (f). Photographs are representative of at least three individual experiments. **p < 0.01 vs. control cells, #p < 0.05, ##p < 0.01 vs. high glucose + NC inhibitor-treated cells. NC: negative control.

Article Snippet: Human retinal microvascular endothelial cells (HRMECs) were purchased from Angio-Proteomie (Boston, MA, USA) and cultured in endothelial cell medium (ECM, Sciencell) supplemented with 5% fetal bovine serum (FBS, Sciencell), 100 U/mL penicillin and 100 μg/mL streptomycin at 37 °C under 5% CO2 and 95% ambient air.

Techniques: Migration, Wound Healing Assay, Expressing, Western Blot, Control, Negative Control

Journal: Bioscience, biotechnology, and biochemistry

Article Title: Inhibition of miR-21-5p suppresses high glucose-induced proliferation and angiogenesis of human retinal microvascular endothelial cells by the regulation of AKT and ERK pathways via maspin.

doi: 10.1080/09168451.2018.1459179

Figure Lengend Snippet: Figure 5. Effects of PI3K/AKT and ERK on the angiogenesis of HRMECs. Cell migration was detected by wound healing assay (a, b). Expression of MMP-2 and MMP-9 were measured by western blot (c). Tube formation and branching points were determined (d, e). Expression of HIF-1α and VEGF were measured by western blot (f). Photographs are representative of three individual experiments. **p < 0.05, **p < 0.01 vs. high glucose-treated cells.

Article Snippet: Human retinal microvascular endothelial cells (HRMECs) were purchased from Angio-Proteomie (Boston, MA, USA) and cultured in endothelial cell medium (ECM, Sciencell) supplemented with 5% fetal bovine serum (FBS, Sciencell), 100 U/mL penicillin and 100 μg/mL streptomycin at 37 °C under 5% CO2 and 95% ambient air.

Techniques: Migration, Wound Healing Assay, Expressing, Western Blot

Journal: Advanced Healthcare Materials

Article Title: A Human‐Based Skin‐Lymphoreticular Model‐on‐Chip to Emulate Inflammatory Skin Conditions

doi: 10.1002/adhm.202503170

Figure Lengend Snippet: Integration of lymphatic endothelial cells (LEC) increases the biomimicry of a self‐assembled lymphoreticular (LR) model. A) Exemplary histological staining of a human lymph node. Scale bar = 1 mm. Copyright permission has been obtained from Xinxiang Happy Science Co., Ltd. B) Schematic illustration of the layering approach. C) Brightfield image of a self‐assembled LR model. D) Representative H&E staining and E) IF images showing the FRC‐derived ECM marker ER‐TR7 (red), DAPI (blue) and the localization of GFP‐tagged LECs of LR models generated using different layering approaches: FLF (FRCs + GPF‐LECs + FRC), FLFN (FRC + GPF‐LECs + (FRC + naïve CD4 + T cells)), FLFA (FRC + GPF‐LECs + (FRC + activated CD4 + T cells)), FLN (FRC + GPF‐LECs + naïve CD4 + T cells), and FLA (FRC + GPF‐LECs + activated CD4 + T cells). Circles and arrows indicate the compartmentalization within LR models. Semi‐quantification of F) ER‐TR7 expression and G) GFP‐LEC distribution in the LR models generated using the different layering approaches. n = 3. One‐way ANOVA followed with Tukey post‐hoc tests were performed. Mean ± SD. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, ****p ≤ 0.0001. H) IF staining against collagen type 1 and fibronectin as key ECM components of lymphoid tissue in FLFA models. Cell nuclei are counterstained with DAPI (blue). Scale bars = 100 µm.

Article Snippet: Green fluorescent protein‐expressing dermal primary human lymphatic endothelial cells (GFP – LECs) (cAP‐0003GFP, male donor) and the Quick Coating Solution (cAP‐01) were purchased from Angio‐Proteomie.

Techniques: Staining, Derivative Assay, Marker, Generated, Expressing

Journal: Science advances

Article Title: Engineering edgeless human skin with enhanced biomechanical properties.

doi: 10.1126/sciadv.ade2514

Figure Lengend Snippet: Fig. 5. Vascularization of WHCs for further transplantation. (A) Illustration of EC seeding protocol in WHCs; the ECs are introduced inside the scaffold through injection and are allowed to attach for 1 hour on each surface before restarting the perfusion. (B) IF whole-mount staining showing FB and EC organization on the inner surface of the WHC; FBs and ECs are both stained with vimentin (red). (C) IF whole-mount staining showing the EC aggregates networking on the WHC inner surface. The EGFP- tagged HDBECs (green) were stained with CD31 (red), a specific endothelial surface marker. (D) Magnification of (C) showing the details of the aggregates sprouting toward each other; the spiral organization of the ECs in the aggregates fades into a very straight orientation in the sprouts. (E) IF staining showing a capillary surrounded by FBs; the capillary stained for CD31 (green) forms close interactions with the vimentin-positive FBs (red) and mimics their overall orientation. Scale bars, 1 mm (B and C), 200 μm (D), and 100 μm (E). Part of the illustration (A) was designed with Biorender.

Article Snippet: 9, eade2514 (2023) 27 January 2023 10 of 16 ow nloaded from https://w w w .science.org on January 23, 2024 The normal adult HDBECs were purchased from Promocell (#C12225, used for prevascularization of hindlimb grafts), while the green fluorescent protein (GFP)–tagged HDBECs were purchased from Angioproteomie (#cAP-0005GFP, used in vitro to assess the development of the blood vasculature in our model).

Techniques: Transplantation Assay, Injection, Staining, Marker